BLNK is a cytoplasmic linker or adaptor protein that plays a critical role in B cell development.
This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions.
The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation.
Mutations in its gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked.
Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 3-5ug/mlELISA: 1:32,000Optimal dilutions to be determined by the researcher.
Positive Control:Daudi Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human BLNK.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the C-terminus of BLNK. Species sequence homology: mouse, rat, canine, porcine and bovine.
