Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis.
Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis.
Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules.
The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules.
May counteract a default induction of apoptosis in G2/M phase.
The acetylated form represses STAT3 transactivation of target gene promoters.
May play a role in neoplasia.
Inhibitor of CASP3 and CASP7.
Isoform 2 and isoform 3 do not appear to play vital roles in mitosis.
Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:MOLT4 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human BIRC5 at 16.4kD.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the C-terminus of BIRC5. Species sequence homology: feline, canine, porcine and bovine.
