VASH1 was originally identified as an endothelium-derived vascular endothelial growth factor (VEGF)-inducible angiogenesis inhibitor that acts in a negative feedback manner.
VASH1 mRNA is expressed brain, heart, kidney and placenta in the adult, with robust expression in various organs during embryonic development.
VASH1 expression in tumor cells and tumor vasculature is silenced by methylation by increased expression of Zeste homolog 2 (EZH2).
The increase of EZH2 expression is induced by VEGF stimulation, leading to poor clinical prognosis.
Recombinant adenovirus expressing VASH prevented tumor angiogenesis and inhibited tumor growth, suggesting that it may be a potentially valuable antitumor therapy in the clinic.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human VASH1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 19aa from near the C-terminus of human VASH1.
