The leucine-rich repeat FLI-I-interacting protein 2 (LRRFIP2), like the related protein LRRFIP1, was identified in a yeast two-hybrid system through binding to the LRR domain of human FLI.
It can activate the Wnt signaling pathway in cultured cells and is thought to be a component of the Wnt signaling pathway that modulates Wnt signaling through interactions with Disheveled to increase the cellular levels and transcription activity of b-catenin.
LRRFIP2 has recently been characterized as a positive regulator of the TLR4 signaling pathway for activating NF-kB during the early host response to LPS stimulation through binding to the TLR adaptor protein MyD88, and that this interaction with MyD88 is governed by phosphorylation of specific residues in LRRFIP2.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Colon Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human LRRFIP2. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa from near the C-terminus of human LRRFIP2.
