84-2113-88　IDH1 (Isocitrate Dehydrogenase [NADP] Cytoplasmic, IDH, Cytosolic NADP-isocitrate Dehydrogenase, IDP, NADP(+)-specific ICDH, Oxalosuccinate Decarboxylase, PICD) 100ug　146682

特徴

• Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.
• These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).
• Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial.
• The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.
• The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production.
• Defects in IDH1 are involved in the development of glioma.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human spleen Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.