C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases.
Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2.
These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:3T3 (NIH) Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human C-raf. Species Crossreactivity: mouse and rat.
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 19aa from near the N-terminus of human C-raf.
