The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension.
Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p.
Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g.
energy metabolism, and thereby are associated with aging and several age-related diseases.
SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues.
SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Immunofluorescence; 20ug/mlWestern Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:I1906-10: IgY, Chicken (HRP) Pab Rb x Ch I1906-12: IgY, Chicken (FITC) Pab Rb x Ch I1906-14: IgY, Chicken (AP) Pab Rb x Ch
Positive Control:Mouse liver tissue lysate and rat lung tissue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human SIRT1. Species Crossreactivity: mouse and rat
Isotype：IgY
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 19aa from near the N-terminus of human SIRT1.
