The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers.
Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder.
BCAS3 is thought to be an estrogen receptor (ER)-a co-activator that acts through PELP1, another ER-a that in turn activates BCAS3 expression.
This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer.
BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BCAS3. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 18aa from near the N-terminus of human BCAS3.
