VLK was identified as a novel protein kinase that was induced after the differentiation of cultured embryonic stem cells into mesendoderm.
It has no homologs in invertebrates, but is highly conserved in vertebrate species although it does not belong to any known protein kinase groups.
VLK is initially expressed in E-cadherin-positive anterior visceral endoderm and mesendoderm, but its expression is later confined to E-cadherin-negative mesenchyme.
It is enriched in the Golgi apparatus and is thought to regulate the rate of protein export from the Golgi.
Targeted disruption of VLK in mice leads to a defect in lung development and neonatal lethality.
It has been suggested that mutations in VLK may be associated with the allergic condition atopy.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Lung Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human VLK. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 15aa from human VLK.
