The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development.
RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family.
Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity.
In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:K562 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human RHBDD1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 14aa from near the C-terminus of human RHBDD1.
