84-2112-45　FRMPD4 (FERM and PDZ Domain-containing Protein 4, PDZ Domain-containing Protein 10, PSD-95-interacting Regulator of Spine Morphogenesis, Preso, KIAA0316, PDZD10, PDZK10) 100ug　146539

特徴

• The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain.
• FRMPD4, also known as Preso, also contains another protein interaction domain termed WW (domain with two conserved Trp residues) at its amino terminus.
• It was identified through a yeast two-hybrid screen using the PDZ domain of PSD-95 as bait and is highly expressed in multiple regions of the brain and is enriched in the postsynaptic density (PSD) fractions.
• Overexpression of FRMPD4 in cultured hippocampal neurons significantly increased the linear density of dendritic spines without changing their length and width; conversely, knockdown experiments using RNAi caused a decrease in spine density, indicating FRMPD4 positively regulates dendritic spine density but not morphology.
• The decreased level of FRMPD4 also resulted in reduced levels of excitatory synaptic transmission, suggesting that FRMPD4 is required for maintenance of excitatory synaptic transmission.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:SK-N-SH Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.