84-2112-41　SQSTM1 (Sequestosome-1, EBI3-associated Protein of 60kD, EBIAP, p60, ORCA, OSIL, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62) 100ug　146535

特徴

• SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination.
• It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1.
• SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain.
• SQSTM1s UBA domain is necessary for recruitment of polyubiquitin and aggresome formation.
• SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.
• Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Spleen Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.