84-2112-39　Mimitin (NDUFA12L, Mimitin, Mitochondrial, B17.2-like, Myc-induced Mitochondrial Protein, NADH Dehydrogenase [Ubiquinone] 1 alpha Subcomplex Assembly Factor 2, NDUFA12-like Protein, FLJ22398) 100ug　146533

特徴

• Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC).
• Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells.
• Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kB-related pathway.
• It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis.
• Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.
• Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Raji Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.