Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle.
The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis.
One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar.
These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage.
CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Kidney Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human CENPO. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa from human CENPO.
