RUSC1, also known as NESCA, shares with the related protein RUSC2 a common domain structure of RUN, leucine zipper and SH3 domain in addition to over 30% aa identity.
RUSC1 is an adapter protein that can bind to the TrkA receptor and is necessary in the NGF-induced neurite growth of PC12 cells.
RUSC1 has also been shown to interact with IkappaB kinase- (IKK-) gamma, the regulatory subunit of the IKK complex that is required for NF-kappaB activation in many signaling pathways such as TNF-R or the TLR pathways.
RUSC1 can also bind to the E3 ubiquitin ligase TRAF6, which then catalyzes RUSC1 polyubiquitination.
Since overexpression of RUSC1 strongly inhibits TRAF6-mediated polyubiquitination of IKK-gamma, RUSC1 may be a link in the IKK-gamma-mediated NF-kappaB activation pathway.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A20 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human RUSC1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa from near the C-terminus of human RUSC1.
