HAAO (3-Hydroxyanthranilate 3,4-dioxygenase) is a monomeric cytosolic protein of the family of intramolecular dioxygenases containing non-heme ferrous iron.
It is widely distributed in peripheral organs, such as liver and kidney, and is present in low amounts in the central nervous system.
This enzyme participates in tryptophan metabolism.
It employs one cofactor, iron.
HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid.
QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors.
Increased cerebral levels of QUIN may participate in the pathogenesis of neurological and inflammatory disorders.
HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.
Furthermore, recent study shows that HAAO are excellent candidate biomarkers for detecting ovarian cancer.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Liver Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human HAAO. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa from near the N-terminus of human HAAO.
