84-2111-70　THEM4 (Thioesterase Superfamily Member 4, Acyl-coenzyme A Thioesterase THEM4, Acyl-CoA Thioesterase THEM4, Carboxyl-terminal Modulator Protein, CTMP) 100ug　146463

特徴

• THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells.
• Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer.
• Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase.
• Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program.
• The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Liver Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.