Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders.
Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration.
SPG15 is the second gene known to be responsible for ARHSP-TCC in the Italian population.
Mutations in this gene are associated with autosomal recessive spastic paraplegia-15.
SPG15 encodes a protein containing a FYVE zinc finger binding domain which is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane.
SPG15 mRNA is widely distributed in human tissues, as well as in rat embryos, suggesting a possible role for this protein during embryonic development.
SPG15 colocalizes partially with endoplasmic reticulum and endosome markers, suggesting a role in intracellular trafficking.
Multiple isoforms of SPG15 are known to exist.
Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Heart Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human SPG15. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa from near the C-terminus of human SPG15.
