Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism.
The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades.
Recent evidence suggests that it may play key roles in regulating apoptosis and aging.
Beta actin is one of six different actin isoforms which have been identified.
Like GAPDH, actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：chicken
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide
Specificity：Recognizes human beta-actin. Species Crossreactivity: mouse and rat
Isotype：IgY
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa from near the C-terminus of human beta-actin. Species Sequence Homology: sheep, rabbit, porcine, equine, chicken, guinea pig and bovine; 100%
