84-2110-65　PIGY, ID (Phosphatidylinositol N-acetylglucosaminyltransferase Subunit Y, Phosphatidylinositol-glycan Biosynthesis Class Y Protein, PIG-Y) 100ug　146358

特徴

• Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum.
• The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2.
• PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI.
• The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
• This antibody only detects isoform 1 of PIG-Y.
• Despite its predicted molecular weight, PIG-Y often migrates at 28-30kD in SDS-PAGE.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Spleen Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.