Disp2 is the second of three known homologs of the D.
melanogaster protein Dispatched.
It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells.
Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the Disp proteins have been implicated in these pathways.
Recently, it has been shown that Disp2 is translationally regulated by the microRNA miR-214 in zebrafish.
Expression of this miRNA decreased Disp2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by Disp2 mutants.
At least two isoforms of Disp2 are known to exist.
This Disp2 antibody is predicted to not cross-react with Disp1 or Disp3.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Disp2. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 19aa from near the C-terminus of human Disp2.
