Wnt10a is a member of the Wnt family, a gene family that encodes secreted signaling proteins that play crucial roles in normal development such as regulation of cell fate and patterning during embryogenesis as well as neoplastic transformation.
Elevated levels of Wnt10a have been detected in several human tumors and cancer cell lines including colorectal cancer and chronic lymphocytic leukemia and it has been suggested that uncontrolled Wnt signaling may contribute to the defect in apoptosis that characterizes these malignancies.
Recently, a mutation in Wnt10a was found to be associated with odonto-onycho-dermal dysplasia, an autosomal recessive ectodermal dysplasia.
Despite the high homology of Wnt10a to Wnt10b, this Wnt10a antibody will not cross-react with Wnt10b.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:RAW264.7 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Wnt10a. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 14aa from near the C-terminus of human Wnt10a.
