Upregulated obese product 11 (UROP11) was isolated from subtractive library for mouse hypothalamus.
It is detected in numerous tissues and is upregulated in the hypothalamus and adipocytes in obese mice.
UROP11 is also observed in human neural and lymphoid tissues; its expression appears to be regulated by the level of lymphoid activation.
Urop11 expression is strongly upregulated both in vivo in mouse hypothalamus and in vitro in mouse neural cell lines after treatment with leptin, indicating Urop11 is a target of leptin activity and thus may play a role in obesity.
Despite its predicted molecular weight, UROP11 often migrates at a much higher apparent size in SDS-PAGE.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Mouse Spleen Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse UROP11. Species Crossreactivity: human and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 12aa from near the internal region of mouse UROP11.
