84-2109-67　AMBRA1, NT (Activating Molecule in BECN1-regulated Autophagy Protein 1, KIAA1736, WDR94) 100ug　146261

特徴

• Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells.
• It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components.
• Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1.
• Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay.
• Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death.
• Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy.
• Multiple isoforms of Ambra1 are known to exist.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/ml, detects a band of ~160kD in rat brain cell lysateImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Rat Brain Tissue Lysate or Human Brain Tissue Slide
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.