BAP3 was initially identified through interaction in a yeast two-hybrid system with the brain-specific angiogenesis inhibitor 1, a p53-target gene that encodes a seven-span transmembrane protein member of the secretin receptor family.
BAP3 is predominantly expressed in the brain and possess high homology with Munc13 and synaptotagmin, suggesting that BAP3 may play a role in regulating neurotransmitter release.
Recent experiments have shown that BAP3 is induced in certain tumors such as desmoplastic small round cell tumor.
Ectopic expression of BAP3 in tumor cells dramatically enhances growth in low serum conditions and colony formation in soft agar, suggesting that the regulated exocytotic pathway may play a role in cancer cell proliferation.
BAP3 is known to have two isoforms; this BAP3 antibody will recognize only isoform 2.
Lower molecular weight bands may represent cleavage or degradation products.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:SK-N-SH Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BAP3.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from near the N-terminus of human BAP3.
