84-2109-41　FBXL20, NT (F-box/LRR-repeat Protein 20, F-box and Leucine-rich Repeat Protein 20, F-box/LRR-repeat Protein 2-like, FBL2, MGC15482) 100ug　146233

特徴

• Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40aa F-box motif.
• SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases.
• Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane.
• Scrapper has orthologs in C.
• elegans, D.
• melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species.
• Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission.
• This identifies protein degradation as a mechanism for holding synaptic communication in check.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-1ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:A20 Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.