84-2109-34　SLC9A1, CT (Sodium/hydrogen Exchanger 1, Na(+)/H(+) Exchanger 1, NHE-1, Solute Carrier Family 9 Member 1, Na(+)/H(+) Antiporter, Amiloride-sensitive, APNH, APNH1, NHE1) 100ug　146226

特徴

• The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters.
• Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton.
• This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins.
• A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger.
• It is also thought that Nhe-1 play a role in hypertension.
• At least two isoforms of Nhe-1 are known to exist.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (FFPE): 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Rat Kidney Tissue Lysate, Human Kidney Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.