Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors.
TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies.
These include TEMs such as TEM1, TEM5, TEM7 and TEM8.
TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells.
TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once.
TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon.
Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2-4ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Rat Kidney Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human TEM5. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to 14aa from near the center of human TEM5.
