84-2109-12　RAP80, NT (BRCA1-A Complex Subunit RAP80, Receptor-associated Protein 80, Retinoid X Receptor-interacting Protein 110, Ubiquitin Interaction Motif-containing Protein 1, UIMC1, RXRIP110) 100ug　146204

特徴

• RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR).
• Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation.
• Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair.
• Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1.
• At least four isoforms of RAP80 are known to exist.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:293 Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.