84-2108-89　IL33, ID (Interleukin-33, IL-33, Interleukin-1 Family Member 11, IL-1F11, Nuclear Factor From High Endothelial Venules, NF-HEV, C9orf26, IL1F11, NFHEV) 100ug　146182

特徴

• Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1a/b, IL-1Ra and IL-18.
• Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and IL-1R accessory protein (IL-1RAcP).
• Receptor binding of IL-33 activates NF-kappaB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6.
• Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine.
• Recent experiments have shown that IL-33 can also colocalize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
• Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE.
• Applications:Suitable for use in Immunofluorescence, ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Lymph Node Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.