BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C.
elegans neuronal cell polarity regulator SAD1.
BRSK2 is expressed in the brain and to a lesser extent in the testes.
BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1.
More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization.
While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth.
Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites.
These neurites also displayed both axonal and dendritic markers.
BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis.
At least four isoforms of BRSK2 are known to exist.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human Brain Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BRSK2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 23aa from near the C-terminus of human BRSK2.
