84-2108-69　BRSK1, ID (BR Serine/Threonine-protein Kinase 1, SAD1 Kinase, Serine/Threonine-protein Kinase SAD-B, KIAA1811, SAD1) 100ug　146160

特徴

• BRSK1 was initially identified as a mammalian homolog to the fission yeast S.
• pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C.
• elegans neuronal cell polarity regulator SAD1.
• BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes.
• Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest.
• Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function.
• More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization.
• While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth.
• Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites.
• These neurites also displayed both axonal and dendritic markers.
• At least two isoforms of BRSK1 are known to exist.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Human Brain Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.