84-2108-52　TMED10, CT (Transmembrane emp24 Domain-containing Protein 10, 21kD Transmembrane-trafficking Protein, S31III125, S31I125, Tmp-21-I, Transmembrane Protein Tmp21, p23, p24 Family Protein delta-1, p24delta, TMP21) 100ug　146143

特徴

• TMP21 is a ubiquitously expressed protein that is involved in vesicular targeting and protein transport.
• More recent experiments have shown that it is also a component in the presenilin complex and modulates the y-secretase but not the e-secretase cleavage activity of the amyloid precursor protein.
• The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1.
• Together, these proteins cleave the amyloid precursor protein at what is known as the y- and e-sites and can lead to the accumulation of the Ab cleavage product that is associated with Alzheimer's disease.
• Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex.
• Suppression of TMP21 expression by siRNA in transfected cells caused increased y-secretase activity but not e-secretase activity, and increased Ab production, demonstrating that TMP21 can modulate y-secretase activity.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Raji Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.