DNA double strand breaks represent a major threat to an organism's genome.
Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death.
The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2.
This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression.
In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest.
Multiple isoforms of ATM are known to exist.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Daudi Cell Lysate or Human Lymph node Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human ATM.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 14aa from near the C-terminus of human ATM.
