Bcl-2-associated athanogene 1 (BAG-1) was first identified as an anti-apoptotic bcl-2-binding protein.
Later it was found to bind the molecular chaperones Hsp70 and Hsc70 through its carboxy-terminal sequence (termed the Bag domain), resulting in the inhibition of the refolding activity of these chaperones.
It is thought that by binding and inhibiting these molecular chaperones, BAG-1is able to modulate the expression level of proteins requiring chaperones to fold correctly.
One such group of proteins that are affected is glucocorticoid receptors.
Other reports have suggested that the level of BAG-1 expression correlates with the aggressiveness of various cancers.
Multiple isoforms of BAG-1 are known to exist.
Applications:Suitable for use in Immunohistochemistry, Immunofluorescence, ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunohistochemistry (paraffin): 5ug/mlImmunohistochemistry: 20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:PC-3 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BAG1. Species Crossreactivity: rat and mouse.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 14aa from near the C-terminus of human BAG-1.
