84-2108-03　BIK (Bcl 2 Interacting Killer, BCL-2-interacting Killer, Apoptosis Inducer NBK, BIP1, BP4, NBK) 100ug　146093

特徴

• Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases.
• Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes.
• One such protein BIK was recently identified as an endoplasmic reticulum (ER)-residing pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family that stimulates mitochondrial release of cytochrome c following p53 induction of apoptosis.
• A significant fraction of BIK is found as an ER transmembrane protein, with most of the protein facing the cytosol.
• Restricting BIK to the ER membrane by replacing the transmembrane region with that of the ER-selective membrane anchor of cytochrome b(5) resulted in a decreased cytochrome c release from mitochondria and a corresponding drop in cell death.
• Recent evidence suggests that BIK cooperates with NOXA, another BH3-only protein, to somehow enhance the activation of Bax to stimulate the rapid release of cytochrome c from mitochondria.
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunocytochemistry: 1ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Jurkat Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.