Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases.
Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes.
Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes..
Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes.
The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression.
At least two isoforms of BIF are known to exist.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BIF. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 15aa from near the C-terminus of human BIF.
