Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family.
These two proteins are highly homologous and can form homo- and heterodimers.
Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18.
It may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria.
Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis.
The p20 fragment of Bap31, when expressed ectopicially, is also a potent inducer cell death.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Ramos Cell Lysate or Rat Lung Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BAP31. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 17aa from near the C-terminus of human BAP31.
