The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP).
IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response.
A novel member in the IRAK/Pelle family has been identified and designated IRAK2.
Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation.
IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).
Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response.
Unlike human IRAK2, murine IRAK2 exists as four alternately spliced isoforms (IRAK2a-d), with two isoforms (IRAK2c and d) acting in an inhibitory fashion.
IRAK2 is expressed in a variety of tissues.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 1ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:RAW264.7 Cell Lysate or A-20 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse IRAK2a. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 17aa from near the C-terminus of mouse IRAK2a.
