84-2107-69　EIF4EBP1 (Eukaryotic Translation Initiation Factor 4E-binding Protein 1, 4E-BP1, eIF4E-binding Protein 1, Phosphorylated Heat- and Acid-stable Protein Regulated by Insulin 1, PHAS-I) 100ug　146058

特徴

• The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms.
• One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors.
• One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs.
• Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation, at least in part by inhibiting the activity of TOR towards 4E-BP1.
• Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2.5-5ug/mlImmunocytochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:3T3 Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.