Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival.
While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak.
Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types.
Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals.
Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S.
In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 10ug/mlImmunocytochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Raji Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Mcl-1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa from near the C-terminus of human Mcl-1.
