Caspase-7, a 35kD cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) is a member of the ICE/Ced-3 subfamily.
Caspase-7 is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits.
Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumor necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADP-ribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1-2ug/mlImmunohistochemistry (Paraffin): 2ug/ml; Does not require protein digestion pre-treatment of paraffin embedded sections e.g.
trypsin or pronase prior to staining.
Does not require antigen retrieval using heat treatment prior to staining.
Optimal dilutions to be determined by the researcher.
IHC Positive Control:Skeletal Muscle Tissue
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes an epitope within the C-terminal region (CT) of human Caspase-7. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to 16aa from near the C-terminus of human Caspase-7.
