Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies.
Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.
Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18.
Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis.
Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Ramos Cell Lysate or A20 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Caspase-2. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 16aa from human Caspase-2 at N-terminal.
