Bruton's tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked ay-globulinaemia, and has since been shown to involved in a number of signaling pathways in hemapoietic lineage.
It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9.
The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens.
BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction.
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 10ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:U937 Cell Lysate or Daudi Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human BTK. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 16aa from near the N-terminus of human BTK.
