SIGIRR is a member of the Toll-like receptor-interleukin 1 receptor superfamily.
Members of this family are defined by the presence of an intracellular Toll-IL-1R (TIR) domain.
The Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses.
SIGIRR was originally identified through database analysis and was shown to have only one Ig domain as opposed to the normal three Ig folds seen in the TIR family.
Similar to ST2, another TIR family member, it has been shown to negatively regulate IL-1 receptor and Toll-like receptor signaling.
However, SIGIRR inhibits TLR-IL-1R signaling by dimerizing with TLR4, TLR5, TLR9, and IL-1R.
It also associates with the down-stream TLR signaling proteins IRAK and TRAF6 in an IL-1-dependent fashion.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A549 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes mouse SIGIRR. Species Crossreactivity: human.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 15aa from near the C-terminus of mouse SIGIRR.
