Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.
Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2.
Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed.
Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1.
Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunocytochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human Bcl-B.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 16aa from near the N-terminus of human Bcl-B.
