84-2107-17　PAK4 (Serine/Threonine-protein Kinase PAK 4, p21-activated Kinase 4, PAK-4, KIAA1142) 100ug　146005

特徴

• The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac.
• They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding.
• Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding.
• The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility.
• Much less is known about the second group, which includes PAK4, 5 and 6.
• These proteins are not activated by Cdc42/Rac binding.
• PAK4 was initially identified as a novel effector of Cdc42Hs.
• Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization.
• Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development.
• PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1-2ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:SW480 Cell Lysate or Human Colon Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.