84-2107-16　PAK5 (p21 Activated Kinase 5, p21-activated Kinase 5, PAK5, PAK-5, KIAA1264, MGC26232, p21(CDKN1A)-activated Kinase 7, p21-activated Kinase 7, PAK7, PAK-7, Protein Kinase PAK5, Serine/threonine-protein Kinase PAK 7) 100ug　146004

特徴

• The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac.
• They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding.
• Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding.
• The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility.
• Much less is known about the second group, which includes PAK4, 5 and 6.
• These proteins are not activated by Cdc42/Rac binding.
• PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both.
• Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth.
• Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.
• Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2-4ug/mlImmunocytochemistry: 2ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:T24 Cell Lysate or K562 Cell Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.