The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac.
They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding.
Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding.
The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility.
Much less is known about the second group, which includes PAK4, 5 and 6.
These proteins are not activated by Cdc42/Rac binding.
PAK6 was initially identified as an androgen receptor in a yeast two hybrid screen and was found to be highly expressed in testis and prostate tissues.
Later experiments have shown it to be activated by MAP kinase kinase 6 and p38 MAP kinase, suggesting that PAK6 may play a role in the cellular response to stress-related signals.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1-4ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlImmunofluorescence: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Raji Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PAK6.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to 13aa from human PAK6.
