84-2107-13　BCL2L13, IN (Bcl-2-like Protein 13, Bcl2-L-13, Protein Mil1, Bcl-rambo, MIL1, CD003) 100ug　146001

特徴

• Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
• Disruption of this process has been implicated in a variety of diseases such as cancer.
• Members of the Bcl-2 family are known to be critical regulators of this process.
• These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains.
• A novel, widely expressed member termed Bcl-rambo was recently identified.
• This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2.
• Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250aa insertion.
• This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.
• Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2-4ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:K562 Cell Lysate or Human Heart Tissue Lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.